Publications

2025

Drawing of a fly superimposed with a barcode

Blanco Mendana, J., Donovan, M., Gengelbach, L., Auch, B., Garbe, J., Gohl, D.M. 2025. Deterministic Genetic Barcoding for Multiplexed Behavioral and Single-Cell Transcriptomic Studies. eLife. doi: https://doi.org/10.7554/eLife.88334.3

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Figure from McCartney et al. 2025

 

McCartney, M.A., Mallez, S., Anderson, S., Garbe, J., Hernández-Elizárraga, V.H., Gohl, D.M. 2025. Population genomics traces zebra mussel invasions along the expanding western North American front in Minnesota, USA to their source waters. Scientific Reports. 15, 43137. doi:https://doi.org/10.1038/s41598-025-27300-6

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Comparison of PCA plots for amplicon and shotgun mycobiome data

Heisel, T., Gonia, S., Dillon, A., Hoops, S.L., Al-Ghalith, G.A., Gohl, D., Mukhopadhyay, S., Puopolo, K., Kennedy, P., Sadowsky, M.J., Knights, D., Johnson A.J., Gerber, J.S., Gale, C.A. 2025. Gut mycobiome maturation and its determinants during early childhood: a comparison of ITS2 amplicon and shotgun metagenomic sequencing approaches. Frontiers in Microbiology. doi:https://doi.org/10.3389/fmicb.2025.1539750.

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2024

Cover image for Alegria and Joshi, Genetics, 2024

 

Alegria, A.D.*, Joshi, A.S.*, Blanco Mendana, J., Khosla, K., Smith, K.T., Auch, B., Donovan, M., Bischof, J., Gohl, D.M., Kodandaramaiah, S.B. 2024. High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot. Genetics. doi: https://doi.org/10.1093/genetics/iyae025

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Image from 2024 Forry et al. Scientific Reports

Forry, S.P., Servetas, S.L., Kralj, J.G., Soh, K., Hadjithomas, M., Carlin, M., de Amorim, M.G., Auch, B., Bakker, M.G., Bartelli, T.F., Bustamante, J.P., Cassol, I., Chalita, M., Dias-Neto, E., Gohl, D.M., Haruna, M.T., Kazantseva, J., Menzel, P., Moda, B.S.,  Neuberger-Castillo, L., Nunes, D., Patel, I.R., Peralta, R.D., Saliou, A., Schwarzer, R., Sevilla, S., Takenaka, I.K.T.M., Wang, J., Knight, R., Gevers, D., Jackson, S.A. 2024. Variability and Bias in Microbiome Metagenomic Sequencing: an Interlaboratory Study Comparing Experimental Protocols. Scientific Reports. 14:9785. doi: https://doi.org/10.1038/s41598-024-57981-4

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2023

Image from 2023 Tan et al. BMC Microbiology

Tan, A.*, Murugapiran, S.*, Mikalauskas, A.*, Koble, J., Kennedy, D., Hyde, F., Ruotti, V., Law, E., Jensen J., Schroth, G.P., Macklaim, J.M., Kuersten, S., Le François, B., Gohl, D.M. 2023. Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomes. BMC Microbiology. 21:299. doi: https://doi.org/10.1186/s12866-023-03037-y

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Image from 2023 iScience Genetic Biocontrol review

Hernández Elizárraga, V.H., Ballantyne, S., O'Brien, L.G., Americo, J.A., Suhr, S.T., Senut, M-C., Minerich, B., Merkes, C.M., Edwards, T.M., Klymus, K., Richter, C.A., Waller, D.L., Passamaneck, Y.J., Rebelo, M.F., Gohl, D.M. 2023. Towards Invasive Mussel Genetic Biocontrol: Approaches, Challenges, and Perspectives. iScience. 108027. https://doi.org/10.1016/j.isci.2023.108027

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Image from 2023 Born et al. Micropublications

Born, G., Bieli, D., Metzler, M., Gohl, D.M., Affolter, M, Müller, M. 2023. No apparent role for the Wari insulator in transcriptional regulation of the endogenous white gene of Drosophila melanogaster. microPublication Biology, doi: https://doi.org/10.17912/micropub.biology.000702

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Image from 2023 Hughes et al. Nutrients

 

Hughes, R.L., Frankenfeld, C. L., Gohl, D.M., Huttenhower, C., Jackson, S.A., Vandeputte, D., Vogtmann, E., Comstock, S.S., Kable, M.E. 2023. Methods in Nutrition & Gut Microbiome Research: An American Society for Nutrition Sattelite Session [13 October 2022]. Nutrients. 15, 2451. doi: https://doi.org/10.3390/nu15112451

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2022

Cover image from McCartney et al 2023, G3

 

McCartney, M.A., Auch, B., Kono, T., Mallez, S., Zhang, Y., Obille, A., Becker, A., Abrahante, J., Garbe, J., Badalamenti, J., Herman, A., Mangelson, H., Liachko, I., Sullivan, S., Sone, E.D., Koren, S., Silverstein, K., Beckman, K., Gohl, D.M. 2022. The genome of the zebra mussel, Dreissena polymorpha: a resource for comparative genomics, invasion genetics, and biocontrol. G3 (Genes, Genomes, Genetics). doi: https://doi.org/10.1093/g3journal/jkab423

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Figure from Holm et al. 2020. Frontiers.

Holm, I., Nardini, L., Pain, A., Bischoff, E., Anderson, C.E., Zongo, S., Guelbeogo, W.M., Sagnon, N., Gohl, D.M., Nowling, R.J., Vernick, K.D., Riehle, M.M. 2022. Comprehensive genomic discovery of non-coding transcriptional enhancers in the African malaria vector Anopheles coluzzii. Frontiers in Genetics. doi: https://doi.org/10.3389/fgene.2021.785934

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2021

Figure from Gohl et al. 2021. NAR.

 

Gohl, D.M., Auch, B., Certano, A., LeFrançois, B., Bouevitch, A., Doukhanine, E., Fragel, C., Macklaim, J., Hollister, E., Garbe, J., Beckman, K.B. 2021. Dissecting and Tuning Primer Editing by Proofreading Polymerases. Nucleic Acids Reseach. gkab471. doi: https://doi.org/10.1093/nar/gkab471

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Figure from Joshi et al. 2021. IEEE.

Joshi, A.S*., Alegria, A.D*., Auch, B., Khosla, K., Blanco Mendana, J., Liu, K., Bischof, J., Gohl, D.M., Kodandaramaiah, S.B. 2021. Multiscale, multi-perspective imaging assisted robotic microinjection of 3D biological structures. 43rd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), doi: https://doi.org/10.1109/EMBC46164.2021.9630858

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Figure from Johnson et al. 2021. Microbiology Spectrum.

Johnson, A.J., Zhou, S., Hoops, S.L., Hillmann, B., Schomaker, M., Kincaid, R., Daniel, J., Beckman, K., Gohl, D.M., Yohe, S., Knights, D., Nelson, A.C. 2021. Saliva testing is accurate for early-stage and presymptomatic COVID-19. Microbiology Spectrum. doi: https://doi.org/10.1128/Spectrum.00086-21

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Figure from Colonetta et al. 2021. Genetics.

Colonnetta, M.M., Abrahante, J.E., Schedl, P., Gohl, D.M., Deshpande, G. 2021. CLAMP regulates zygotic genome activation in Drosophila embryos. Genetics. iyab107. doi: https://doi.org/10.1093/genetics/iyab107

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2020

Figure from Gohl et al. 2020. BMC Genomics.

Gohl, D.M., Garbe, J., Grady, P., Daniel, J., Watson, R.H.B., Nelson, A., Yohe, S., Beckman, K.B. 2020. A Rapid, Cost-Effective Tailed Amplicon Method for Sequencing SARS-CoV-2. BMC Genomics. 21:863. doi: https://doi.org/10.1186/s12864-020-07283-6

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Figure from Nelson et al. 2020. bioRxiv.

Nelson, A.C., Auch, B., Schomaker, M., Gohl, D.M., Grady, P., Johnson, D., Kincaid, R., Karnuth, K.E., Daniel, J., Fiege, J.K., Fay, E.J., Bold, T., Langlois, R.A., Beckman, K.B., Yohe, S. 2020. Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods. bioRxiv. doi: https://doi.org/10.1101/2020.04.02.022186

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2019

Figure from Gohl et al. 2019. Genome Biology.

Gohl, D.M., Magli, A., Garbe, J., Becker, A., Johnson, D., Anderson, S., Auch, B., Billstein, B., Froehling, E., McDevitt, S.L., Beckman, K.B. 2019. Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification. Genome Biology. 20(1):85.

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Figure from Minato et al. 2019. mSystems.

 

Minato, Y., Gohl D.M., Thiede, J.M., Chacón, J.M., Harcombe, W.R., Maruyama, F., Baughn, A.D. 2019. Genome-wide assessment of Mycobacterium tuberculosis conditionally essential metabolic pathways. mSystems. 4:e00070-19.

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Figure from Nardini et al. 2019. Scientific Reports.

Nardini, L., Holm, I., Pain, A., Bischoff, E., Gohl, D.M., Zongo, S., Guelbeogo, W.M., Sagnon, N., Vernick, K.D., Riehle, M.M. 2019. Influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector Anopheles coluzzii. Scientific Reports. 9:15275. doi: https://doi.org/10.1038/s41598-019-51730-8

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Figure from McCartney et al. 2019. Conservation Biology.

 

 

McCartney, M.A., Mallez, S., Gohl, D.M. 2019. Genome projects in invasion biology. Conservation Genetics. https://doi.org/10.1007/s10592-019-01224-x

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2018

Figure from Hillman et al. 2018. mSystems.

 

Hillmann, B., Al-Ghalith, G.A., Shields-Cutler, R., Zhu, Q., Gohl, D.M., Beckman, K.B., Knight, R., Knights, D. 2018. Evaluating the Information Content of Shallow Shotgun Metagenomics. mSystems. 3(6): https://doi.org/doi:10.1128/mSystems.00069-18

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Figure from Song et al. 2018. Phytobiomes.

Song, Z., Schlatter, D., Gohl, D.M., Kinkel, L.L. 2018. Run-to-Run Sequencing Variation Can Introduce Taxon-Specific Bias in the Evaluation of Fungal Microbiomes. Phytobiomes. https://doi.org/10.1094/PBIOMES-09-17-0041-R

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Figure from Castle et al. 2018. Phytobiomes.

Castle, S.C., Song, Z., Gohl, D.M., Gutknecht, J.L.M., Rosen, C.J., Sadowsky, M.J., Samac, D.A., Kinkel, L.L. 2018. DNA Template Dilution Impacts Amplicon Sequencing-Based Estimates of Soil Fungal Diversity. Phytobiomes. 2(2):100-107. https://doi.org/10.1094/PBIOMES-09-17-0037-R

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Figure from Dale et al. 2018. mSystems.

 

Dale, J.L., Beckman, K.B., Willett, J.L.E., Nilson, J.L., Palani, N.P., Baller, J.A., Hauge, A., Gohl, D.M., Erickson, R., Manias, D.A., Sadowsky, M.J., Dunny, G.M. 2018. Comprehensive Functional Analysis of the Enterococcus faecalis Core Genome Using an Ordered, Sequence-Defined Collection of Insertional Mutations in Strain OG1RF. mSystems. 3(5): e00062-18. http://doi.org/10.1128/mSystems.00062-18

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Figure from Liou et al. 2018. Nature Communications.

Liou, N-F., Lin, S-H., Chen, Y-J., Tsai, K-T., Yang, C-J., Lin, T-Y., Wu, T-H., Lin, H-J., Chen, Y-T., Gohl, D., Silies, M., Chou, Y-H. 2018. Diverse populations of local interneurons integrate into the Drosophila adult olfactory circuit. Nature Communications. 9:2232.

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Figure from Burgos et al. 2018. eLife.

Burgos, A., Honjo, K., Ohyama, T., Qian, C. S., Shin, G. J., Gohl, D. M., Silies, M., Tracey, D.W., Zlatic, M., Cardona, A., Grueber, W. B. 2018. Nociceptive interneurons control modular motor pathways to promote escape behavior in Drosophila. eLife. 7:e26016. http://doi.org/10.7554/eLife.26016

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2017

Figure from Fisher et al. 2017. eLife.

 

Fisher Y.E., Yang, H.H, Isaacman-Beck, J., Xie, M., Gohl, D.M., Clandinin, T.R. 2017. FlpStop, a tool for conditional gene control in Drosophila. eLife. 6:e22279.

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Figure from Rawson et al. 2017. Journal of Molecular Biology.

Rawson, J.M.O., Gohl, D.M., Landman, S.R., Roth, M.E., Meissner, M.E., Peterson, T.S., Hodges, J.S., Beckman, K.B., Mansky, L.M. 2017. Single strand consensus sequencing reveals that HIV type but not subtype significantly impacts viral mutation frequencies and spectra. Journal of Molecular Biology. https://doi.org/10.1016/j.jmb.2017.05.010

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Figure from Belda et al. 2017. Scientific Reports.

Belda, E., Coulibaly, B., Fofana, A., Beavogui, A.H., Traore, S.F., Gohl, D.M., Vernick, K.D, Riehle, M.M. 2017. Preferential suppression of Anopheles gambiae host sequences allows detection of the mosquito eukaryotic microbiome. Scientific Reports. 7:3421.

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Figure from Sickmann et al. 2017. D.I.S.

 

Sickmann, M., Gohl, D. M., Affolter, M., Müller, M. 2017. The Drosophila melanogaster straw locus is allelic to laccase2. Drosophila Information Service. 100:171-174.

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Figure from Gohl. 2017. Nature Biotechnology.

 

Gohl, D.M. 2017. The ecological landscape of microbiome science. Nature Biotechnology. 35:1047-1049.

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Figure from Gohl et al. 2017. Book Chapter.

 

 

Gohl, D.M., Morante, J., Venken, K.J.T. 2017. The Current State of the Neuroanatomy Toolkit in the Fruit Fly Drosophila melanogaster. In: Decoding Neural Circuit Structure and Function. Editors: Mathias Wernet and Arzu Celik. Springer International Publishing.

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2016

Figure from Gohl et al. 2016. Nature Biotechnology.

 

Gohl, D.M., Vangay, P., Garbe, J., MacLean, A., Hauge, A., Becker, A., Gould, T.J., Clayton, J.B., Johnson, T.J., Hunter, R., Knights, D., Beckman, K.B. 2016. Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies. Nature Biotechnology. 34(9):942-949.

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2015

Figure from Fisher et al. 2015. Current Biology.

 

Fisher, Y.E., Leong, J.C.S., Sporar, K., Ketkar, M.D., Gohl, D.M., Clandinin, T.R., Silies, M.A. 2015. A class of visual neurons with wide-field properties is required for local motion detection. Current Biology. 25(24):3178-3189.

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Figure from Bieli et al. 2015. PLOS Genetics.

 

Bieli, D., Kanca, O., Requena, D., Hamaratoglu, F., Gohl, D., Schedl, P., Affolter, M., Slattery, M., Müller, M., Estella, C. 2015. Establishment of a developmental compartment requires interactions between three synergistic cis-regulatory modules. PLOS Genetics. 11(10):e1005376.

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Figure from Bieli et al. 2015. G3.

 

Bieli, D., Kanca, O., Gohl, D., Denes, A., Schedl, P., Affolter, M., Slattery, M., Müller, M. 2015. The Drosophila melanogaster Mutants apblot and apXasta Affect an Essential apterous Wing Enhancer. G3 (Genes, Genomes, Genetics). 5(6):1129–1143.

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Figure from Baldwin et al. 2015. PeerJ.

Baldwin, E.A., Walther-Antonio, M., MacLean, A.M., Gohl, D.M., Beckman, K.B., Chen, J., White, B., Creedon, D.J., Chia, N. 2015. Persistent microbial dysbiosis in preterm premature rupture of membranes from onset until delivery. PeerJ. 3:e1398.

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2014

Figure from Gohl et al. 2014. Genetics.

 

Gohl, D.M.*, Freifeld, L.*, Silies, M., Horowitz, M., Clandinin, T.R. 2014 Large-scale mapping of transposable element insertion sites using digital encoding of DNA sample identity. Genetics. 196(3):615-623.

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Figure from Velez et al. 2014. Journal of Neurogenetics.

 

Velez, M.M. , Gohl, D. , Clandinin, T.R., Wernet, M.F. 2014. Differences in neural circuitry guiding behavioral responses to polarized light presented to either the dorsal or ventral retina in Drosophila. Journal of Neurogenetics. 28(3-4):348-360.

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Figure from Silies et al. 2014. Annual Review of Neuroscience.

 

Silies, M.A., Gohl, D.M., Clandinin, T.R. 2014. Motion-Detecting Circuits in Flies: Coming into View. Annual Review of Neuroscience. 37:307-327.

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2013

Figure from Silies et al. 2013. Neuron.

 

 

Silies, M.*, Gohl, D.M.*, Fisher, Y.E., Freifeld, L., Clark D.A., Clandinin, T.R. 2013. Modular use of peripheral input channels tunes motion-detecting circuitry. Neuron. 79(1):111-117.

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Figure from Clark et al. 2013. Nature Neuroscience.

Clark, D.A.*, Fitzgerald, J.E.*, Ales, J.M.*, Gohl, D.M., Silies, M., Norcia, A.M., and Clandinin,T.R. 2013. Flies and humans use a shared computational strategy that exploits natural scene statistics to estimate motion. Nature Neuroscience. 17(2):296-303.

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Figure from Gao et al. 2013. Current Biology.

 

 

 

Gao, X.J., Potter, C.J., Gohl, D.M., Silies, M., Katsov, A.Y., Clandinin, T.R. and Luo, L. 2013. Specific Kinematics and Motor-Related Neurons for Aversive Chemotaxis in Drosophila. Current Biology. 23(13):1163-1172.

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2012

Figure from Burke et al. 2012. Nature.

 

 

Burke, C.J., Huetteroth, W., Owald, D., Perisse, E., Krashes, M.J., Das, G., Gohl, D., Silies, M., Certel, S., and Waddell, S. 2012. Layered reward signaling through octopamine and dopamine in Drosophila. Nature. 492(7429):433-7.

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Figure from Manzo et al. 2012. PNAS.

 

 

Manzo, A., Silies, M., Gohl, D.M., Scott, K. 2012. Motor neurons controlling fluid injestion in Drosophila. Proceedings of the National Academy of Sciences. 109(16):6307-12.

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Figure from Gohl et al. 2012. Book Chapter.

 

Gohl, D.M., Silies, M.A., Clandinin, T.R. 2012. Drosophila as a genetic model to investigate motion vision. In: The Making and Unmaking of Neural Circuits in Drosophila. Editor: Bassem Hassen. Humana Press.

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2011

Figure from Gohl et al. 2011. Nature Methods.

 

 

Gohl, D.M., Silies, M.A., Gao, X.J., Bhalerao, S., Luongo, F.J., Lin, C-C., Potter, C.J., Clandinin, T.R. 2011. A versatile in vivo system for directed dissection of gene expression patterns. Nature Methods. 8:231-237.

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Figure from Gohl et al. 2011. Genetics.

 

 

Gohl, D., Aoki, T., Blanton, J., Shanower, G., Kappes, G., Schedl, P. 2011. Mechanism of boundary action: roadblock, sink, or loop? Genetics. 187(3):731-748.

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2008

Figure from Gohl et al. 2008. Genetics.

 

 

Gohl, D., Müller, M., Pirrotta, V., Affolter, M., Schedl, P. 2008. Enhancer blocking and transvection at the Drosophila apterous locus. Genetics. 178(1):127-143.

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2004

Figure from Schweinsberg et al. 2004.

 

Schweinsberg, S., Hagstrom, K., Gohl, D., Schedl, P., Kumar, R.P., Mishra, R.., Karch F. 2004. The enhancer blocking activity of the Fab-7 boundary from the Drosophila bithorax complex requires GAGA-factor binding sites. Genetics. 168(3):1371-1384.

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